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pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3
(Plasmid #127356)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127356 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniTurbo (BirA mutant)
  • Species
    BirA from E. coli, codon optimized for human and mutated
  • Insert Size (bp)
    771
  • Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, I305V
  • Promoter no promoter
  • Tags / Fusion Proteins
    • GS linker (N terminal on insert)
    • V5 (N terminal on insert)
    • GS linker (C terminal on insert)
    • mVenus (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    miniTurbo was obtained from Alice Ting's lab at Stanford University (cloned from V5-miniTurbo-NES_pCDNA3, Addgene catalog #107170).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3 was a gift from Dominique Bergmann (Addgene plasmid # 127356 ; http://n2t.net/addgene:127356 ; RRID:Addgene_127356)
  • For your References section:

    Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID. Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC. Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. 10.7554/eLife.47864 PubMed 31535972