Proximity labeling of protein complexes and cell type-specific organellar proteomes in Arabidopsis enabled by TurboID.
Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. PubMed Article
Mair A, Xu SL, Branon TC, Ting AY, Bergmann DC
Elife. 2019 Sep 19;8. pii: 47864. doi: 10.7554/eLife.47864. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127349 | pENTR_L1-YFP-Turbo-NES-L2 | gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins |
| 127350 | pENTR_L1-YFP-Turbo-L2 | gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins |
| 127351 | pENTR_L1-YFP-miniTurbo-NES-L2 | gateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteins |
| 127352 | pENTR_L1-YFP-miniTurbo-L2 | gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins |
| 127353 | pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3 | gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins |
| 127354 | pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3 | gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins |
| 127355 | pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3 | gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins |
| 127356 | pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3 | gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins |
| 127357 | pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2 | gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice |
| 127358 | pENTR_L1-Turbo-NES-YFP-STOP-L2 | gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice |
| 127359 | pENTR_L1-miniTurbo-NES-YFP-STOP-L2 | gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice |
| 127360 | pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2 | gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice |
| 127361 | pENTR_L1-Turbo-YFP-NLS-STOP-L2 | gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice |
| 127362 | pENTR_L1-miniTurbo-YFP-NLS-STOP-L2 | gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice |
| 127363 | R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP | Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants |
| 127365 | R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS | Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants |
| 127366 | R4pGWB601_UBQ10p-Turbo-NES-YFP | Binary vector for expressing cytosolic TurboID-YFP under the UBQ10 promoter in plants |
| 127368 | R4pGWB601_UBQ10p-Turbo-YFP-NLS | Binary vector for expressing nuclear TurboID-YFP under the UBQ10 promoter in plants |
| 127369 | R4pGWB601_UBQ10p-miniTurbo-NES-YFP | Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants |
| 127370 | R4pGWB601_UBQ10p-miniTurbo-YFP-NLS | Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants |