pMH-HT_SsCHAD-H29A-R32A-R36A-R69A
(Plasmid
#127787)
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PurposeExpresses a SsCHAD mutant protein in E. coli cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 127787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMH-HT
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Backbone manufacturerMichael Hothorn
- Backbone size w/o insert (bp) 5313
- Total vector size (bp) 5949
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSsCHAD
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Alt nameQ97YW1
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SpeciesSulfolobus solfataricus
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Insert Size (bp)636
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Mutationmutations in His29, Arg32, Arg36 and Arg69 to Ala
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Entrez GeneSULM_RS11045 (a.k.a. SULM_RS11045, SULM_11050)
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Tag
/ Fusion Protein
- 6xHis tag + TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TGCGTCCGGCGTAGAGGATCGAGATCT
- 3′ sequencing primer CAGCTTCCTTTCGGGCTTTGTTAGCAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-HT_SsCHAD-H29A-R32A-R36A-R69A was a gift from Michael Hothorn (Addgene plasmid # 127787 ; http://n2t.net/addgene:127787 ; RRID:Addgene_127787) -
For your References section:
Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Lorenzo-Orts L, Hohmann U, Zhu J, Hothorn M. Life Sci Alliance. 2019 May 27;2(3). pii: 2/3/e201900385. doi: 10.26508/lsa.201900385. Print 2019 Jun. 10.26508/lsa.201900385 PubMed 31133615