Addgene - DCT.VV.Orf92 Plasmid Data

Plasmid 12780: DCT.VV.Orf92

Gene/insert name:
B1R
Alt name:
protein kinase
Insert size:
903
Species:
Vaccinia Virus
GenBank ID:
9791130
Vector backbone:
pcDNA 3.1D V5-His-TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5514
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
Universal T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Jack Bennink
Principal Investigator:
Jon Yewdell
Terms and Licenses:
MTA

Comments:

Vaccinia Virus ORF library, ORF 92

MW (kDa): 34.3. Time of expression: early. From McCraith et al: "essential, protein kinase, virion protein , ts: C2, C3, C25, P: SFV H2R, H: various protein kinases". Forward primer: ATGAACTTTCAAGGACTTGTGTTAA. Reverse primer: TTAATAATATACACCCTGCATTAATATGTG.

Protein sequence: mnfqglvltdncknqwvvgpligkggfgsiyt tndnnyvvkiepkangslfteqafytrvlkps vieewkkshnikhvglitckafglyksinvey rflvinrlgadldavirannnrlpkrsvmlig ieilntiqfmheqgyshgdikasnivldqidk nklylvdyglvskfmsngehvpfirnpnkmdn gtleftpidshkgyvvsrrgdletlgycmirw lggilpwtkisetkncalvsatkqkyvnntat llmtslqyaprellqyitmvnsltyfeepnyd efrhilmqgvyy

Additional reference: see McCraith et al, PNAS 2000
97:4879-4884. Genome-wide analysis of vaccinia virus protein-protein
interactions.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12780" in your Materials and Methods section.