pCambia2300 - ProRUBY:RUBY-Citrine
(Plasmid
#128796)
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PurposeFluorescent reporter for subcellular localisation and expression analysis of RUBY
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCambia2300
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Backbone manufacturerCambia
- Backbone size w/o insert (bp) 8711
- Total vector size (bp) 15884
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Vector typePlant Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRUBY PARTICLES IN MUCILAGE
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Alt nameRUBY
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)6128
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GenBank IDNM_101845.3
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Entrez GeneAT1G19900 (a.k.a. AT1G19900, F6F9.4, F6F9_4)
- Promoter ProRUBY
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Tag
/ Fusion Protein
- Citrine (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer caggaaacagctatgac
- 3′ sequencing primer gcatcgagctgaagggcat (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCambia2300 - ProRUBY:RUBY-Citrine was a gift from George Haughn (Addgene plasmid # 128796 ; http://n2t.net/addgene:128796 ; RRID:Addgene_128796) -
For your References section:
RUBY, a Putative Galactose Oxidase, Influences Pectin Properties and Promotes Cell-To-Cell Adhesion in the Seed Coat Epidermis of Arabidopsis. Sola K, Gilchrist EJ, Ropartz D, Wang L, Feussner I, Mansfield SD, Ralet MC, Haughn GW. Plant Cell. 2019 Apr;31(4):809-831. doi: 10.1105/tpc.18.00954. Epub 2019 Mar 8. 10.1105/tpc.18.00954 PubMed 30852555
Map uploaded by the depositor.
