Purpose(Empty Backbone) Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||128845||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSen Wu, Capecchi laboratory
Vector typeMammalian Expression, Mouse Targeting
Growth in Bacteria
Copy numberLow Copy
A portion of this plasmid was derived from a plasmid made byDTA negative selection cassette is from Philippe Soriano. p15A origin backbone is from Sen Wu, Capecchi laboratory.
Terms and Licenses
Positive and negative selection enable efficient gene targeting in mouse embryonic stem cells.
A typical workflow is to subclone a genomic DNA fragment from a BAC clone using pStart-K #20346, then Gateway-clone (with LR Clonase II) the DTA negative selection cassette in this plasmid.
Different positive selection cassettes are available on Addgene, for example, pFNF #22687.
This plasmid is derived from pWS-TK6 #20350 and pSABgeolox2DTA #13447. The TK negative selection cassette was replaced by DTA negative selection cassette.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDTA-5 was a gift from Mario Capecchi (Addgene plasmid # 128845 ; http://n2t.net/addgene:128845 ; RRID:Addgene_128845)