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pcDNA3-EGFP-RhoA-wt
(Plasmid #12965)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pcDNA3-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    RhoA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    582
  • GenBank ID
    L25080
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, RHO12, RHOH12)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 for EGFP
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-EGFP-RhoA-wt was a gift from Gary Bokoch (Addgene plasmid # 12965)
  • For your References section:

    Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu K, Bokoch GM, Hahn KM. J Biol Chem. 2000 Mar 31. 275(13):9725-33. 10.1074/jbc.275.13.9725 PubMed 10734125