Addgene - pcDNA3-EGFP-RhoA-wt Plasmid Data

Plasmid 12965: pcDNA3-EGFP-RhoA-wt

Gene/insert name:
RhoA
Insert size:
582
Species:
H. sapiens (human)
GenBank ID:
L25080
Entrez Gene:
RHOA (ARH12, ARHA, RHO12, RHOH12)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3-EGFP
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6100
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 for EGFP List of Sequencing Primers
3' sequencing primer:
Sp6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Gary Bokoch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas. Subauste et al (J Biol Chem. 2000 Mar 31. 275(13):9725-33. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12965" in your Materials and Methods section.