Addgene - pcDNA3-TLR1-YFP Plasmid Data

Plasmid 13014: pcDNA3-TLR1-YFP

Gene/insert name:
Toll-Like Receptor 1
Alt name:
TLR1
Alt name:
CD281
Alt name:
TIL
Insert size:
2358
Species:
H. sapiens (human)
GenBank ID:
BC109093
Entrez Gene:
TLR1 (CD281, TIL, TIL. LPRS5, rsc786)
Fusion protein or tag:
YFP
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3-YFP
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6100
Cloning site 5':
BamHI / BglII
Site destroyed during cloning:
Yes
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
GTCTTGTAGTTGCCGTCGTC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (3)
Principal Investigator:
Doug Golenbock
Terms and Licenses:
MTA

Comments:

The primer for sequencing out the 5' end of the GFP based constructs (GFP/EGFP, CFP, YFP) is 5'- GTCTTGTAGTTGCCGTCGTC -3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13014" in your Materials and Methods section.