pJPF0190
(Plasmid #130268)

• Purpose: Piggybac vector for the expression of mRuby2-fused human PRKACA
• Depositing Lab: Hana El-Samad
• Publication: Fonseca et al bioRxiv 2020, 747030

Backbone

• Vector backbone: pPB
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 7600
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRKACA
• Alt name: Protein Kinase A Catalytic Subunit alpha
• Species: H. sapiens (human)
• Insert Size (bp): 1053
• Entrez Gene: PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
• Promoter: CAG
• Tag / Fusion Protein:
  • mRuby2 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7_fwd

Resource Information

• Supplemental Documents:
  • jpf0190_ppbj_puro-cag-prkacamruby2.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://www.addgene.org/23495/ is where PRKACA was originally cloned from.

Please visit https://www.biorxiv.org/content/10.1101/747030v2 for bioRxiv preprint.