Addgene - pcDNA3-YFP Plasmid Data

Plasmid 13033: pcDNA3-YFP

Gene/insert name:
Yellow fluorescent protein
Alt name:
YFP
Alt name:
EYFP
Insert size:
720
Vector backbone:
pcDNA3
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5446
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
GTCTTGTAGTTGCCGTCGTC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
YFP from discontinued clonetech vector.
Sequence: View sequences (3)
Principal Investigator:
Doug Golenbock
Terms and Licenses:
MTA

Comments:

The primer for sequencing out the 5' end of the GFP based constructs (GFP/EGFP, CFP, YFP) is 5'- GTCTTGTAGTTGCCGTCGTC -3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13033" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

13030: pcDNA3-CFP

13032: pcDNA3-mRFP

13031: pcDNA3-EGFP

13018: pcDNA3-TLR4-YFP

13642: pcDNA3-TLR9-YFP