Addgene - 28 pCS plcg 1 Plasmid Data

Plasmid 13059: 28 pCS plcg 1

Gene/insert name:
plcg1
Insert size:
3938
Species:
D. rerio (zebrafish)
Entrez Gene:
plcg1 (fj46f04, wu:fj46f04, wu:fj57a05)
Vector backbone:
pCS2+
(Search Vector Database)
Backbone size w/o insert (bp):
4100
5' sequencing primer:
Sp6 List of Sequencing Primers
3' sequencing primer:
EBV rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Lawson et al (Genes Dev. 2003 Jun 1. 17(11):1346-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13059" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

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