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Plasmid 13059: 28 pCS plcg 1
  • plcg1

  • 3938

  • D. rerio (zebrafish)

  • plcg1 (fj46f04, wu:fj46f04, wu:fj57a05)

  • pCS2+
    (Search Vector Database)

  • 4100

  • Sp6 List of Sequencing Primers

  • EBV rev

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Nathan Lawson

  • UBMTA

Comments: 

The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Lawson et al (Genes Dev. 2003 Jun 1. 17(11):1346-51. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13059" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only