28 pCS plcg 1
(Plasmid #13059)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    plcg1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3938
  • Entrez Gene
    plcg1 (a.k.a. fj46f04, wu:fj46f04, wu:fj57a05)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    28 pCS plcg 1 was a gift from Nathan Lawson (Addgene plasmid # 13059)
  • For your References section:

    phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Lawson ND, Mugford JW, Diamond BA, Weinstein BM. Genes Dev. 2003 Jun 1. 17(11):1346-51. 10.1101/gad.1072203 PubMed 12782653