Addgene - 41 pBS119 d10L Plasmid Data

Plasmid 13065: 41 pBS119 d10L

Gene/insert name:
fli genomic
Insert size:
7017
Species:
D. rerio (zebrafish)
Entrez Gene:
fli1a (fli, fli1, cb855, fli-1, wu:fc45b11)
Vector backbone:
na
(Search Vector Database)
Backbone size w/o insert (bp):
2950
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

An XbaI fragment of the zebrafish fli1 promoter that includes 1 kb upstream of exon 1 and 6 kb of intron 1 was subcloned into pBluescript from a PAC obtained by hybridization to the 5' end of the fli1 cDNA. An oligonucleotide linker containing NheI, SrfI, and AscI sites was cloned into an MluI site upstream of the fli1 start codon to give pBS119d10L.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Lawson et al (Development. 2001 Oct . 128(19):3675-83. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13065" in your Materials and Methods section.