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41 pBS119 d10L
(Plasmid #13065)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13065 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    fli genomic
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    7017
  • Entrez Gene
    fli1a (a.k.a. cb855, fli, fli-1, fli1, wu:fc45b11)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

An XbaI fragment of the zebrafish fli1 promoter that includes 1 kb upstream of exon 1 and 6 kb of intron 1 was subcloned into pBluescript from a PAC obtained by hybridization to the 5' end of the fli1 cDNA. An oligonucleotide linker containing NheI, SrfI, and AscI sites was cloned into an MluI site upstream of the fli1 start codon to give pBS119d10L.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    41 pBS119 d10L was a gift from Nathan Lawson (Addgene plasmid # 13065)
  • For your References section:

    Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, Weinstein BM. Development. 2001 Oct . 128(19):3675-83. PubMed 11585794