362 pCS Cherry DEST
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13075||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5778
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsMust be grown in a strain resistant to ccdB.
Copy numberHigh Copy
Insert Size (bp)752
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
There is a H30R mutation in mCherry. This mutation does not affect plasmid function and the construct has been validated for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:362 pCS Cherry DEST was a gift from Nathan Lawson (Addgene plasmid # 13075)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311