Addgene - pCSDest Plasmid Data

Plasmid 22423: pCSDest

Gene/insert name:
None
Vector backbone:
pCS2+
(Search Vector Database)
Backbone size (bp):
5800
Cloning site 5':
StuI
Site destroyed during cloning:
Unknown
Cloning site 3':
StuI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F, SP6 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin and Chloramphenicol
Growth strain(s)
ccdB Survival
Growth temperature (℃):
37
Growth instructions:
ccdB Survival
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Nathan Lawson
Terms and Licenses:
MTA

Comments:

pCS2+ was digested with Stu I and ligated with the Reading Frame B Gateway cassette

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc et al (Dev Dyn. 2007 Nov . 236(11):3077-87. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22423" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

13070: 221 pCS 3MT DEST

13071: 223 pCS EGFP DEST

13075: 362 pCS Cherry DEST

22424: pCSDest2

22462: p3Eegfp