|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17095||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCS2+ (modified)
- Backbone size (bp) 4103
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
pCS2+ has been modified by the deletion of the AC preceeding the T7 promoter addition of the sequence TTCTGCAGCCC immediately following the XbaI site. The PstI site added to CS2+ is encompassed in the sequence (CTGCAG).
Modifications were made by Robert Davis of Marc Kirshner's lab to improve the efficiency of the transcription from the T7 promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2P+ was a gift from Marc Kirschner (Addgene plasmid # 17095 ; http://n2t.net/addgene:17095 ; RRID:Addgene_17095)