pCS2 mt-GFP (BamHI minus)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15681||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCS2 mt-GFP
- Backbone size (bp) 5100
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Proteins
- 6x Myc (N terminal on backbone)
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
The pCS2 plasmid can be used to synthesize RNA via its SP6 promoter, or directly drive expression in eukarotic cells via the CMV promoter.
In pCS2mt-GFP, the S65T mutant form of GFP has been inserted such that the myc tags and the GFP are in-frame.
You can subclone your favorite sequence between the myc tags and the GFP using EcoRI and Xba I sites. The EcoRI site's AAT defines the reading frame for the insert. This produces an N-terminally myc-tagged, C-terminally GFP-tagged chimeric polypeptide.
Please see lab website for more information (go to Methods -> Green Plasmids): http://spot.colorado.edu/~klym/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2 mt-GFP (BamHI minus) was a gift from Michael Klymkowsky (Addgene plasmid # 15681 ; http://n2t.net/addgene:15681 ; RRID:Addgene_15681)
Map uploaded by the depositor.