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pCS2 mt-GFP (BamHI minus)
(Plasmid #15681)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15681 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • 6x Myc (N terminal on backbone)
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The pCS2 plasmid can be used to synthesize RNA via its SP6 promoter, or directly drive expression in eukarotic cells via the CMV promoter.

In pCS2mt-GFP, the S65T mutant form of GFP has been inserted such that the myc tags and the GFP are in-frame.

You can subclone your favorite sequence between the myc tags and the GFP using EcoRI and Xba I sites. The EcoRI site's AAT defines the reading frame for the insert. This produces an N-terminally myc-tagged, C-terminally GFP-tagged chimeric polypeptide.

Please see lab website for more information (go to Methods -> Green Plasmids): http://spot.colorado.edu/~klym/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 mt-GFP (BamHI minus) was a gift from Michael Klymkowsky (Addgene plasmid # 15681)