pCS2-MT GLI2 FL
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17648||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4350
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)4760
Entrez GeneGLI2 (a.k.a. CJS, HPE9, PHS2, THP1, THP2)
- Promoter CMV IE94
/ Fusion Protein
- 6x Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
There is an R250C mutation in the gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2-MT GLI2 FL was a gift from Erich Roessler (Addgene plasmid # 17648 ; http://n2t.net/addgene:17648 ; RRID:Addgene_17648)
For your References section:A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz AA, Muenke M. Hum Mol Genet. 2005 Aug 1. 14(15):2181-8. 10.1093/hmg/ddi222 PubMed 15994174