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pCS2-MT GLI2 FL
(Plasmid #17648)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17648 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4760
  • Entrez Gene
    GLI2 (a.k.a. CJS, HPE9, PHS2, THP1, THP2)
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • 6x Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There is an R250C mutation in the gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-MT GLI2 FL was a gift from Erich Roessler (Addgene plasmid # 17648 ; http://n2t.net/addgene:17648 ; RRID:Addgene_17648)
  • For your References section:

    A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz AA, Muenke M. Hum Mol Genet. 2005 Aug 1. 14(15):2181-8. 10.1093/hmg/ddi222 PubMed 15994174