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GLI K12
(Plasmid #16419)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16419 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI
  • Alt name
    Gli-1
  • Alt name
    glioma-associated oncogene homolog 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3601
  • Entrez Gene
    GLI1 (a.k.a. GLI, PAPA8, PPD1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GLI K12 was a gift from Bert Vogelstein (Addgene plasmid # 16419 ; http://n2t.net/addgene:16419 ; RRID:Addgene_16419)
  • For your References section:

    The GLI gene is a member of the Kruppel family of zinc finger proteins. Kinzler KW, Ruppert JM, Bigner SH, Vogelstein B. Nature. 1988 Mar 24. 332(6162):371-4. 10.1038/332371a0 PubMed 2832761