pCag SE (Self-excising) FlpO-2A-Cre EV
(Plasmid
#130986)
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Purposeepisomal expression of FlpO and Cre recombinases, self excising
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 130986 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepOG44
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Backbone manufacturerThermoFisher
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Modifications to backboneCMV promoter converted to CAG promoter, F5 sites added for FlpO self-recombination
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Vector typeMammalian Expression, Cre/Lox, Unspecified ; episomal expression vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlpO-2A-Cre
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SpeciesS. cerevisiae (budding yeast); P1 bacteriophage
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Mutationprotamine intron added to FlpO to prevent bacterial self-recombination
- Promoter CMV/Chick β-actin (CAG)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pCAG-F (addgene sequencing primer)
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Self Excising- FlpO and Cre are surrounded by F5 FRT sites but the FlpO contains an intron to prevent bacterial self-recombination during DNA production
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCag SE (Self-excising) FlpO-2A-Cre EV was a gift from Joshua Breunig (Addgene plasmid # 130986 ; http://n2t.net/addgene:130986 ; RRID:Addgene_130986) -
For your References section:
Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, Breunig JJ. Cell. 2019 Sep 19;179(1):251-267.e24. doi: 10.1016/j.cell.2019.08.013. 10.1016/j.cell.2019.08.013 PubMed 31539496
Map uploaded by the depositor.
