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pCag SE (Self-excising) FlpO-2A-Cre EV
(Plasmid #130986)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 130986 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOG44
  • Backbone manufacturer
    ThermoFisher
  • Modifications to backbone
    CMV promoter converted to CAG promoter, F5 sites added for FlpO self-recombination
  • Vector type
    Mammalian Expression, Cre/Lox, Unspecified ; episomal expression vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlpO-2A-Cre
  • Species
    S. cerevisiae (budding yeast); P1 bacteriophage
  • Mutation
    protamine intron added to FlpO to prevent bacterial self-recombination
  • Promoter CMV/Chick β-actin (CAG)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pCAG-F (addgene sequencing primer)
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Self Excising- FlpO and Cre are surrounded by F5 FRT sites but the FlpO contains an intron to prevent bacterial self-recombination during DNA production

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCag SE (Self-excising) FlpO-2A-Cre EV was a gift from Joshua Breunig (Addgene plasmid # 130986 ; http://n2t.net/addgene:130986 ; RRID:Addgene_130986)
  • For your References section:

    Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, Breunig JJ. Cell. 2019 Sep 19;179(1):251-267.e24. doi: 10.1016/j.cell.2019.08.013. 10.1016/j.cell.2019.08.013 PubMed 31539496