PurposeTransient expression of firefly luciferase from the Zea mays ubiquitin promotor
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||131860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4344
- Total vector size (bp) 5997
Vector typePlant Expression
Growth in Bacteria
Insert Size (bp)1653
- Promoter ZmUBQ
- Cloning method Unknown
- 5′ sequencing primer TTAGCCCTGCCTTCATACGC
- 3′ sequencing primer ACCGCGCGCGATAATTTATC (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pZmUBQ:LUC was a gift from Paul Schulze-Lefert (Addgene plasmid # 131860 ; http://n2t.net/addgene:131860 ; RRID:Addgene_131860)
For your References section:A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors. Saur IML, Bauer S, Lu X, Schulze-Lefert P. Plant Methods. 2019 Oct 24;15:118. doi: 10.1186/s13007-019-0502-0. eCollection 2019. 10.1186/s13007-019-0502-0 PubMed 31666804