Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

moPrP(3F4) D177N
(Plasmid #1319)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1319 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcb6
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PrP D177N
  • Alt name
    prion protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    900
  • Mutation
    D177N point mutation
  • GenBank ID
    NM_011170
  • Entrez Gene
    Prnp (a.k.a. AA960666, AI325101, CD230, P, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, S, Sinc, prP27-30, prP33-35C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site bglII, EcoRI, KpnI, NotI, ClaI, EcoRV (not destroyed)
  • 3′ cloning site bamHI??? (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    moPrP(3F4) D177N was a gift from Susan Lindquist (Addgene plasmid # 1319 ; http://n2t.net/addgene:1319 ; RRID:Addgene_1319)
  • For your References section:

    Wild-type PrP and a mutant associated with prion disease are subject to retrograde transport and proteasome degradation. Ma J, Lindquist S. Proc Natl Acad Sci U S A 2001 Dec 18;98(26):14955-60. Epub 2001 Dec 11. 10.1073/pnas.011578098 PubMed 11742063