PurposeReplace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133565||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 9999
Vector typeInsect Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePhiC31 Recombinase
Insert Size (bp)1842
Entrez Geneint (a.k.a. phiC31p51)
- Promoter 20X UAS
- Cloning method Gibson Cloning
- 5′ sequencing primer ATCAGGTCCATGACGTTTCC
- 3′ sequencing primer GGCGCCTAAGAAGAAGAGGA (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJFRC-20XUAS-IVS-PhiC31 was a gift from Tom Clandinin (Addgene plasmid # 133565 ; http://n2t.net/addgene:133565 ; RRID:Addgene_133565)