Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #133817)


Item Catalog # Description Quantity Price (USD)
Plasmid 133817 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Alt name
    Synaptotagmin 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Entrez Gene
    Syt1 (a.k.a. P65)
  • Promoter hSyn (human synapsin I promoter)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Depositor Comments

5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFSW Syt1-C2B IRES GFP was a gift from Edwin Chapman (Addgene plasmid # 133817 ; ; RRID:Addgene_133817)
  • For your References section:

    Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin. Courtney NA, Bao H, Briguglio JS, Chapman ER. Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. 10.1038/s41467-019-12015-w PubMed 31501440