pGEX-4T-1 EGFP-Sumo2 G
(Plasmid #13383)

• Depositing Lab: Mary Dasso
• Publication: Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sumo2 G
• Alt name: SUMO-2
• Species: H. sapiens (human)
• Insert Size (bp): 300
• Mutation: Nonconjugatable SUMO2G: single C-terminal glycine at amino acid position 92
• GenBank ID: NP_008868
• Entrez Gene: SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pEGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry