Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

pFbx15(-544/-527)-TK-Luc
(Plasmid #13414)

Add to Cart
Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pTAL-Luc
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Upstream region of mouse Fbx15 gene
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    20
  • Mutation
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer pGV-2U
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The sequence of primer pGV-2U is ACT AAC ATA CGC TCT CCA TCA A.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFbx15(-544/-527)-TK-Luc was a gift from Shinya Yamanaka (Addgene plasmid # 13414)
  • For your References section:

    Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, Niwa H, Yamanaka S. Mol Cell Biol. 2003 Apr . 23(8):2699-708. 10.1128/MCB.23.8.2699-2708.2003 PubMed 12665572