|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18115||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4622
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNanog homeobox
SpeciesH. sapiens (human)
Insert Size (bp)918
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer cctctagactgccggatctagcta (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMXs is originally from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Terms and Licenses
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-hNANOG (Plath) was a gift from Kathrin Plath (Addgene plasmid # 18115)
For your References section:Generation of human induced pluripotent stem cells from dermal fibroblasts. Lowry WE, Richter L, Yachechko R, Pyle AD, Tchieu J, Sridharan R, Clark AT, Plath K. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8. 10.1073/pnas.0711983105 PubMed 18287077