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pAC-Kan-alphaGal4
(Plasmid #13421)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pACLU-Kan
  • Backbone size w/o insert (bp) 3600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Needs strain with lacIq
  • Copy number
    Low Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    RNAP alpha-Gal4
  • Insert Size (bp)
    900

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-Kan-alphaGal4 was a gift from Keith Joung (Addgene plasmid # 13421)