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Plasmid 13421: pAC-Kan-alphaGal4
  • RNAP alpha-Gal4

  • 900

  • pACLU-Kan
    (Search Vector Database)

  • Bacterial Expression

  • 3600

  • AGA GCC TGA TAA AAA CGG TTA GCG List of Sequencing Primers

  • Kanamycin

  • XL1 Blue

  • 37

  • Needs strain with lacIq

  • Low Copy

  • View sequences (2)
  • View map

  • Keith Joung

  • UBMTA

Comments: 

Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13421" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only