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Plasmid 13421: pAC-Kan-alphaGal4
  • RNAP alpha-Gal4

  • 900

  • pACLU-Kan
    (Search Vector Database)

  • Bacterial Expression

  • 3600

  • AGA GCC TGA TAA AAA CGG TTA GCG List of Sequencing Primers

  • Kanamycin

  • XL1 Blue

  • 37

  • Needs strain with lacIq

  • Low Copy

  • View sequences (2)
  • View map

  • Keith Joung

  • UBMTA

Comments: 

Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13421" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only