Addgene - pAC-Kan-alphaGal4 Plasmid Data

Plasmid 13421: pAC-Kan-alphaGal4

Gene/insert name:
RNAP alpha-Gal4
Insert size:
900
Vector backbone:
pACLU-Kan
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3600
5' sequencing primer:
AGA GCC TGA TAA AAA CGG TTA GCG List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
Growth instructions:
Needs strain with lacIq
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13421" in your Materials and Methods section.