pCAG-CFP-SaCas9-HF (without sgRNA)
(Plasmid
#134470)
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PurposeHuman expression vector for SaCas9-HF variant.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 134470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAG-CFP
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStaphylococcus aureus Cas9 nucleases with high genome-wide specificity
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Alt nameSaCas9-HF
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene
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Terms and Licenses
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Industry Terms
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-CFP-SaCas9-HF (without sgRNA) was a gift from Zongli Zheng (Addgene plasmid # 134470 ; http://n2t.net/addgene:134470 ; RRID:Addgene_134470) -
For your References section:
Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity. Tan Y, Chu AHY, Bao S, Hoang DA, Kebede FT, Xiong W, Ji M, Shi J, Zheng Z. Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1906843116. doi: 10.1073/pnas.1906843116. 10.1073/pnas.1906843116 PubMed 31570596