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pCAG-CFP-SaCas9-HF (without sgRNA)
(Plasmid #134470)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 134470 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Staphylococcus aureus Cas9 nucleases with high genome-wide specificity
  • Alt name
    SaCas9-HF

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-CFP-SaCas9-HF (without sgRNA) was a gift from Zongli Zheng (Addgene plasmid # 134470 ; http://n2t.net/addgene:134470 ; RRID:Addgene_134470)
  • For your References section:

    Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity. Tan Y, Chu AHY, Bao S, Hoang DA, Kebede FT, Xiong W, Ji M, Shi J, Zheng Z. Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1906843116. doi: 10.1073/pnas.1906843116. 10.1073/pnas.1906843116 PubMed 31570596