Addgene - pcDNA3 HA human NEMO Plasmid Data

Plasmid 13512: pcDNA3 HA human NEMO

Gene/insert name:
NEMO
Alt name:
IKKgamma
Insert size:
1260
Species:
H. sapiens (human)
GenBank ID:
NM_003639
Entrez Gene:
IKBKG (AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IP, IP1, IP2, IPD2, NEMO, ZC2HC9)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pcDNA3-HA
(Search Vector Database)
Backbone manufacturer:
Made in Guan Lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Kunliang Guan
Terms and Licenses:
MTA

Comments:

See "Author's Map" for more information.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Negative regulation of the forkhead transcription factor FKHR by Akt. Tang et al (J Biol Chem. 1999 Jun 11. 274(24):16741-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13512" in your Materials and Methods section.