Addgene - pCMV4-3 HA/IkB-alpha Plasmid Data

Plasmid 21985: pCMV4-3 HA/IkB-alpha

Gene/insert name:
I-kappa B alpha
Alt name:
IkB-alpha
Alt name:
NFKBI
Insert size:
1000
Species:
H. sapiens (human)
Entrez Gene:
NFKBIA (IKBA, MAD-3, NFKBI)
Fusion protein or tag:
3x HA
Terminal:
N terminal on backbone
Vector backbone:
pCMV4-3HA
(Search Vector Database)
Backbone manufacturer:
Andersson,S. et al, J. Biol. Chem. 264 (14), 8222-8229 (1989)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4874
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Warner Greene
Terms and Licenses:
MTA

Comments:

Parental vector = pCMV4 3xHA;
3xHA inserted into MluI/HindIII of pCMV4.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Both amino- and carboxyl-terminal sequences within I kappa B alpha regulate its inducible degradation. Sun et al (Mol Cell Biol. 1996 Mar . 16(3):1058-65. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21985" in your Materials and Methods section.