pCMV4 p50
(Plasmid #21965)

• Depositing Lab: Warner Greene
• Publication: Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89(5):1875-9.

Backbone

• Vector backbone: pCMV4
• Backbone manufacturer: Andersson,S. et al, J. Biol. Chem. 264 (14), 8222-8229 (1989)
• Backbone size w/o insert (bp): 4874
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NF-kB p50 subunit
• Alt name: NFKB1
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• GenBank ID: M55643
• Entrez Gene: NFKB1 (a.k.a. CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry