Addgene - pCMV4 p65 Plasmid Data

Plasmid 21966: pCMV4 p65

Gene/insert name:
NFkB p65 subunit
Alt name:
RelA
Insert size:
2500
Species:
H. sapiens (human)
Entrez Gene:
RELA (NFKB3, p65)
Mutation:
This construct was the product of 3 subcloning procedures: 1. XbaI-EcoRI-p65-EcoRI-KpnI from pBluescript II SK +/- was digested with XbaI/KpnI, and subcloned to M13 mp19. 2. HindIII-XbaI-p65-HindIII-KpnI from the M13 mp19 construct was digested with HindIII, then subcloned into pCMV4. 3. The resulting pCMV4 p65 plasmid can be liberated using HindIII, with a size of ~2.5 kb. The large size (p65 is only 1.5kb) is due to the inclusion of multiple polylinkers from pBluescript and M13mp19 as well as non-coding regions of p65.
Vector backbone:
pCMV4
(Search Vector Database)
Backbone manufacturer:
Andersson,S. et al, J. Biol. Chem. 264 (14), 8222-8229 (1989)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4874
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
CMV forward: cgcaaatgggcggtaggcgtg List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (3)
Principal Investigator:
Warner Greene
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The 65-kDa subunit of human NF-kappa B functions as a potent transcriptional activator and a target for v-Rel-mediated repression. Ballard et al (Proc Natl Acad Sci U S A. 1992 Mar 1. 89(5):1875-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21966" in your Materials and Methods section.