flex-ChrimsonR-EYFP-Kv
(Plasmid
#135319)
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PurposeCre-dependent expression of soma-targeted ChrimsonR
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135319 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4827
- Total vector size (bp) 6807
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Modifications to backboneEF1a promoter exchanged for synapsin
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChrimsonR
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SpeciesSynthetic
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Insert Size (bp)1980
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GenBank IDKF992060
- Promoter synapsin
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Tag
/ Fusion Protein
- EYFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer cttTATACGAAGTTatgCTAGcgccaCCatggctgagctgatca
- 3′ sequencing primer ATTATACGAAGTTATGGCGCGCCttaaAACCGCGTAGCCTCTGGAA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byChrimsonR sequence amplified from Addgene plasmid #99231
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2019.12.13.876128v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
flex-ChrimsonR-EYFP-Kv was a gift from Hongkui Zeng (Addgene plasmid # 135319 ; http://n2t.net/addgene:135319 ; RRID:Addgene_135319) -
For your References section:
Synaptic connectivity to L2/3 of primary visual cortex measured by two-photon optogenetic stimulation. Hage TA, Bosma-Moody A, Baker CA, Kratz MB, Campagnola L, Jarsky T, Zeng H, Murphy GJ. Elife. 2022 Jan 21;11:e71103. doi: 10.7554/eLife.71103. 10.7554/eLife.71103 PubMed 35060903
Map uploaded by the depositor.
