|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13532||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2200
/ Fusion Proteins
- See map
- See map
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byVenus is from Dr. Atsushi Miyawaki.
Terms and Licenses
For more information, see
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTK222 was a gift from Wolf Frommer (Addgene plasmid # 13532 ; http://n2t.net/addgene:13532 ; RRID:Addgene_13532)
For your References section:Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB. PLoS Biol. 2007 Oct;5(10):e257. 10.1371/journal.pbio.0050257 PubMed 17896864