Addgene - pCMV5 mouse src K295R Y527F Plasmid Data

Plasmid 13657: pCMV5 mouse src K295R Y527F

Gene/insert name:
src K295R Y527F
Alt name:
c-src
Alt name:
src
Insert size:
2000
Species:
M. musculus (mouse)
Entrez Gene:
Src (RP23-169M4.1, AW259666, pp60c-src)
Mutation:
K295R Y527F. Dominant negative mutant.
Vector backbone:
pCMV5
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
hGH-pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Joan Brugge
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13657" in your Materials and Methods section.