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BPV00605 pET21GG2-His(6)-BECN(1-265)
(Plasmid #136686)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136686 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Beclin-1 IDR + CC
  • Alt name
    Intrinsically disordered region, BH3 motif, and Coiled-coil domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    867
  • Mutation
    DNA optimized for E. coli expression
  • Entrez Gene
    BECN1 (a.k.a. ATG6, VPS30, beclin1)
  • Promoter T7
  • Tag / Fusion Protein
    • Hexa-Histadine (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPV00605 pET21GG2-His(6)-BECN(1-265) was a gift from Colin Garvie (Addgene plasmid # 136686 ; http://n2t.net/addgene:136686 ; RRID:Addgene_136686)
  • For your References section:

    The Autophagy-Related Beclin-1 Protein Requires the Coiled-Coil and BARA Domains To Form a Homodimer with Submicromolar Affinity. Ranaghan MJ, Durney MA, Mesleh MF, McCarren PR, Garvie CW, Daniels DS, Carey KL, Skepner AP, Levine B, Perez JR. Biochemistry. 2017 Dec 26;56(51):6639-6651. doi: 10.1021/acs.biochem.7b00936. Epub 2017 Dec 14. 10.1021/acs.biochem.7b00936 PubMed 29185708