Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
The Autophagy-Related Beclin-1 Protein Requires the Coiled-Coil and BARA Domains To Form a Homodimer with Submicromolar Affinity.
Ranaghan MJ, Durney MA, Mesleh MF, McCarren PR, Garvie CW, Daniels DS, Carey KL, Skepner AP, Levine B, Perez JR
Biochemistry. 2017 Dec 26;56(51):6639-6651. doi: 10.1021/acs.biochem.7b00936. Epub 2017 Dec 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
136684BPV00500 pET21GG2 His(6)-TF-BECN(1-450)Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)
136685BPV00554 pET21GG2-His(6)-BECN(248-450)Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag
136686BPV00605 pET21GG2-His(6)-BECN(1-265)Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag
136688BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

Antibodies from Article