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(Plasmid #136920)


Item Catalog # Description Quantity Price (USD)
Plasmid 136920 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized)
  • Mutation
    see manuscript
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Depositor Comments

Insert has leaky expression during bacterial propagation and can result in a higher than expected spontaneous mutation rate. The insert should always be sequence validated before use.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BE4max-NRCH was a gift from David Liu (Addgene plasmid # 136920 ; ; RRID:Addgene_136920)
  • For your References section:

    Continuous evolution of SpCas9 variants compatible with non-G PAMs. Shannon M. Miller, Tina Wang, Peyton B. Randolph, Mandana Arbab, Max W. Shen, Tony P. Huang, Zaneta Matuszek, Gregory A. Newby, Holly A. Rees & David R. Liu. Nat Biotechnology 10.1038/s41587-020-0412-8