|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||136920||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBR322 CMV
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
SpeciesSynthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized)
- Promoter CMV
- Cloning method Ligation Independent Cloning
Insert has leaky expression during bacterial propagation and can result in a higher than expected spontaneous mutation rate. The insert should always be sequence validated before use.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-BE4max-NRCH was a gift from David Liu (Addgene plasmid # 136920 ; http://n2t.net/addgene:136920 ; RRID:Addgene_136920)
For your References section:Continuous evolution of SpCas9 variants compatible with non-G PAMs. Shannon M. Miller, Tina Wang, Peyton B. Randolph, Mandana Arbab, Max W. Shen, Tony P. Huang, Zaneta Matuszek, Gregory A. Newby, Holly A. Rees & David R. Liu. Nat Biotechnology 10.1038/s41587-020-0412-8