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pAAV-nEF-Con/Foff 2.0-ChR2-mCherry
(Plasmid #137143)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 137143 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV2-nEF-WPRE
  • Backbone size w/o insert (bp) 4600
  • Vector type
    AAV, Cre/Lox, Synthetic Biology ; Flp/FRT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Con/Foff 2.0-ChR2-mCherry
  • Species
    Synthetic; Prokaryotic
  • Insert Size (bp)
    2400
  • Mutation
    H134R
  • Promoter nEF

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer GACCCTGCTTGCTCAACTCT
  • 3′ sequencing primer GGGCCACAACTCCTCATAAA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-nEF-Con/Foff 2.0-ChR2-mCherry was a gift from Karl Deisseroth & INTRSECT 2.0 Project (Addgene plasmid # 137143 ; http://n2t.net/addgene:137143 ; RRID:Addgene_137143)
  • For your References section:

    Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals. Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M, Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 10.1016/j.neuron.2020.06.003. 10.1016/j.neuron.2020.06.003 PubMed 32574559