PurposeLentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138369||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 10043
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert namesequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-basP:fLuc-TK:rLuc was a gift from Nathan Lawson (Addgene plasmid # 138369 ; http://n2t.net/addgene:138369 ; RRID:Addgene_138369)
For your References section:Genomic Characterization of Endothelial Enhancers Reveals a Multifunctional Role for NR2F2 in Regulation of Arteriovenous Gene Expression. Sissaoui S, Yu J, Yan A, Li R, Zhu LJ, Kucukural A, Yukselen O, Lawson ND. Circ Res. 2020 Feb 17. doi: 10.1161/CIRCRESAHA.119.316075. 10.1161/CIRCRESAHA.119.316075 PubMed 32065070