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piB-GFP
(Plasmid #13844)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13844 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TATA-GFP-SV40
  • Alt name
    GFP
  • Insert Size (bp)
    2600

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See map (not destroyed)
  • 3′ cloning site See map (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

piB-GFP is a donor vector that contains an hsp70 TATA box fused to an enhanced GFP open reading frame and an SV40 polyA tail. This gene construct is flanked by inverted attB sites in the vector pBluescript. piB-GFP is a useful vector for cloning a gene of interest into a donor cassette; the GFP gene can be excised and new sequences can be inserted between the inverted attB sites using BamHI, SalI, or HindIII.

*One note on preparing this vector: we had difficulty growing piB-GFP in a larger 25 ml culture for midiprepping, but there were no problems in smaller 3 ml cultures suitable for minipreps. For all of our GFP injections, we used mini-prepped piB-GFP that was further purified on a pcr-purification column to remove RNAse contaminants.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piB-GFP was a gift from Ting Wu (Addgene plasmid # 13844 ; http://n2t.net/addgene:13844 ; RRID:Addgene_13844)
  • For your References section:

    Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Bateman JR, Lee AM, Wu CT. Genetics. 2006 Jun . 173(2):769-77. 10.1534/genetics.106.056945 PubMed 16547094