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Addgene

pCKmatBC
(Plasmid #138587)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138587 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCK01
  • Total vector size (bp) 6735
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
  • Alt name
    matBC
  • Species
    Rhizobium
  • Insert Size (bp)
    2957
  • Mutation
    matB contains native GUG start codon
  • Promoter Plac promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCKmatBC was a gift from Markus Jeschek (Addgene plasmid # 138587 ; http://n2t.net/addgene:138587 ; RRID:Addgene_138587)
  • For your References section:

    Biotin-independent strains of Escherichia coli for enhanced streptavidin production. Jeschek M, Bahls MO, Schneider V, Marliere P, Ward TR, Panke S. Metab Eng. 2017 Mar;40:33-40. doi: 10.1016/j.ymben.2016.12.013. Epub 2017 Jan 3. 10.1016/j.ymben.2016.12.013 PubMed 28062280