pCKmatBC
(Plasmid
#138587)
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PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 138587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCK01
- Total vector size (bp) 6735
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namebicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
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Alt namematBC
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SpeciesRhizobium
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Insert Size (bp)2957
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MutationmatB contains native GUG start codon
- Promoter Plac promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CAGGAAACAGCTATGAC
- 3′ sequencing primer GTAAAACGACGGCCAGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCKmatBC was a gift from Markus Jeschek (Addgene plasmid # 138587 ; http://n2t.net/addgene:138587 ; RRID:Addgene_138587) -
For your References section:
Biotin-independent strains of Escherichia coli for enhanced streptavidin production. Jeschek M, Bahls MO, Schneider V, Marliere P, Ward TR, Panke S. Metab Eng. 2017 Mar;40:33-40. doi: 10.1016/j.ymben.2016.12.013. Epub 2017 Jan 3. 10.1016/j.ymben.2016.12.013 PubMed 28062280