pSBBi-RP GST-TCS1
(Plasmid #139166)

• Purpose: SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
• Depositing Lab: Neal Devaraj
• Publication: Methylxanthine Synthesis in Human Cells (unpublished)

Backbone

• Vector backbone: pSBBi-RP
• Total vector size (bp): 7521
• Vector type: Mammalian Expression ; Transposon
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Glutathione transferase + caffeine synthase 1 [Camellia sinensis]
• Alt name: GST-TCS1
• Alt name: GST-Tea Caffeine Synthase 1
• Alt name: TCS1
• Species: Camellia sinensis
• Insert Size (bp): 1818
• GenBank ID: BAB12278.1
• Tag / Fusion Protein:
  • glutathione transferase (GST) (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pSBBI-RP GST-TCS1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.