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Addgene
Neal Devaraj Lab: Methylxanthine Synthesis in Human Cells
Unpublished

Plasmids from Article

ID Plasmid Purpose
139159pSBBi-RP PcCSSB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana caffeine synthase (PcCS); the other side contains RFP and puromycin resistance genes.
139160pSBBi-RP GST-PcCSSB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes.
139161pSBBi-RP TCS1SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
139162pSBBi-RP CCS1SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
139163pSBBi-RP CCS1(Δ304-316)SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1) CCS-CTS deletion mutant; the other side contains RFP and puromycin resistance genes.
139164pSBBi-RP GST-CCS1SB-transposon with constitutive bi-directional promoter. One side contains N-GST coffee caffeine synthase 1 (GST-CCS1) fusion; the other side contains RFP and puromycin resistance genes.
139165pSBBi-RP CaDXMTSB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes.
139166pSBBi-RP GST-TCS1SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes.
139167pSBBi-RP CsAncCSSB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes.
139168pSBBi-GB CaXMT1SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
139169pSBBi-GB GST-CaXMT1 SB-transposon with constitutive bi-directional promoter. One side contains N-GST Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
139170pSBBi-RP PcAncCS2SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2); the other side contains RFP and puromycin resistance genes.
139171pSBBi-GB TCS2SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS2) from Camellia sinensis; the other side contains GFP and blasticidin resistance genes.
139172pSBBi-RP CaMXMTSB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes.
139176pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMTSB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone.
139184pSB-Bleo STAT3Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
139185pSBBi-Pur GFP-TMSB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.
139189pSB-Bleo Gal4 CCS1SB-transposon with constitutive bleomycin resistance and Gal4-inducible coffee caffeine synthase 1 (CCS1).
139190pSB-Bleo Gal4 tBFPGal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.
158133pSBBi-Pur LaG17-synNotch-TetRVP64Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
158135pSBBi-Pur LaG17-synNotch-Gal4VP64Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

Antibodies from Article