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PurposeDetecting Myo10 by fluorescence microscopy
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139780 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-Myo10
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Backbone manufacturerRichard E. Cheney, UNC
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMyosin X
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Alt nameMYO10
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SpeciesH. sapiens (human)
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Insert Size (bp)6177
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BgIII (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-Myo10WT was a gift from Staffan Stromblad (Addgene plasmid # 139780 ; http://n2t.net/addgene:139780 ; RRID:Addgene_139780) -
For your References section:
PtdIns(3,4,5)P(3) is a regulator of myosin-X localization and filopodia formation. Plantard L, Arjonen A, Lock JG, Nurani G, Ivaska J, Stromblad S. J Cell Sci. 2010 Oct 15;123(Pt 20):3525-34. doi: 10.1242/jcs.069609. 10.1242/jcs.069609 PubMed 20930142
