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Addgene

pLARRY-EGFP
(Plasmid #140025)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140025 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 7712
  • Total vector size (bp) 8432
  • Modifications to backbone
    Inverted cassette (EF1a promoter - unidirectional polyA)
  • Vector type
    Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Alt name
    enhanced green fluorescent protein
  • Species
    Aequorea victoria
  • GenBank ID
    U55762.1
  • Promoter EF1alpha

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer cattctcaagcctcagacagtggtt
  • 3′ sequencing primer gaaggcacaggtcgacaccagt
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLARRY-EGFP was a gift from Fernando Camargo (Addgene plasmid # 140025 ; http://n2t.net/addgene:140025 ; RRID:Addgene_140025)
  • For your References section:

    Lineage tracing on transcriptional landscapes links state to fate during differentiation. Weinreb C, Rodriguez-Fraticelli A, Camargo FD, Klein AM. Science. 2020 Jan 23. pii: science.aaw3381. doi: 10.1126/science.aaw3381. 10.1126/science.aaw3381 PubMed 31974159