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pcDNA5-pEF-piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)-pEF-GalNAc-T2-msGFP2
(Plasmid #140163)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140163 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    GALNT2 (a.k.a. GalNAc-T2)
  • Promoter EF-1a
  • Tag / Fusion Protein
    • ER signal sequence (MGWSCIILFLVATATGAHS) (N terminal on backbone) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Depositor Comments

IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-pEF-piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)-pEF-GalNAc-T2-msGFP2 was a gift from Benjamin Glick (Addgene plasmid # 140163 ; http://n2t.net/addgene:140163 ; RRID:Addgene_140163)
  • For your References section:

    A photostable monomeric superfolder GFP. Valbuena FM, Fizgerald I, Strack RL, Andruska N, Smith L, Glick BS. Traffic. 2020 May 15. doi: 10.1111/tra.12737. 10.1111/tra.12737 PubMed 32415747