AAV-6P-SEW-YC3.6
(Plasmid #31379)

• Depositing Lab: Fritjof Helmchen
• Publication: Minderer et al J Physiol. 2011 Nov 14.

Backbone

• Vector backbone: AAV-6P-SEWB
• Backbone size w/o insert (bp): 4800
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Yellow Cameleon 3.60
• Alt name: YC3.60
• Species: synthetic construct
• Insert Size (bp): 1962
• GenBank ID: AB178712
• Tags / Fusion Proteins:
  • eCFP (N terminal on insert)
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Received from lab of A. Miyawaki
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Optical recording of neuronal activity with a genetically-encoded calcium indicator in anesthetized and freely moving mice.
Lütcke H, Murayama M, Hahn T, Margolis DJ, Astori S, Zum Alten Borgloh SM, Göbel W, Yang Y, Tang W, Kügler S, Sprengel R, Nagai T, Miyawaki A, Larkum ME, Helmchen F, Hasan MT
Front Neural Circuits. 2010 Apr 29;4:9.

There are a few mismatches between Addgene's sequencing results and the full sequence but they do not alter plasmid function. There are two 2 nucleotide deletions corresponding to nucleotides 487-488 and 494-495. There is also a mismatch at position 791. These do not alter protein function.