Addgene - pCMV-GCaMP5G Plasmid Data

Plasmid 31788: pCMV-GCaMP5G

Gene/insert name:
GCaMP5G
Alt name:
GCaMP3-T302L R303P D380Y
Insert size:
1353
Species:
R. norvegicus (rat), G. gallus (chicken)
Species:
A. victoria (jellyfish)
Fusion protein or tag:
6xHis
Terminal:
N terminal on insert
Fusion protein or tag:
T7 epitope
Terminal:
N terminal on insert
Fusion protein or tag:
Xpress tag
Terminal:
N terminal on insert
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3955
Promoter:
CMV
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
CMV Forward List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Jasper Akerboom and Loren L. Looger
Sequence: View sequences (4)
Principal Investigator:
Douglas Kim
Principal Investigator:
Loren Looger
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Optimization of a GCaMP calcium indicator for neural activity imaging. Akerboom et al (J Neurosci. 2012 Oct 3;32(40):13819-40. doi: 10.1523/JNEUROSCI.2601-12.2012. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 31788" in your Materials and Methods section.