|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22692||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Insert Size (bp)1300
MutationN363D mutation (increased fluorescence)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There are a few sequence differences between author sequence and Addgene quality control sequence. The function of the plasmid is not affected.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:G-CaMP3 was a gift from Loren Looger (Addgene plasmid # 22692 ; http://n2t.net/addgene:22692 ; RRID:Addgene_22692)
For your References section:Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, Petreanu L, Akerboom J, McKinney SA, Schreiter ER, Bargmann CI, Jayaraman V, Svoboda K, Looger LL.. Nat Methods. 2009 Dec;6(12):875-81. 10.1038/nmeth.1398 PubMed 19898485